RNA

Part:BBa_K2715018

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-08)


Antisense RNA targeting tcdB 2

Usage

This part is an antisense RNA which targets the Clostridium difficile toxin TcdB. It has been used in the composite part BBa_K2715008.


Biology

The antisense targets 50bp of the coding sequence of C. difficile TcdB as well as the upstream region to include the ribosome binding site.


BBa K2715018 pic.png


Characterisation

This part was characterised as part of a composite part BBa_K2715008 in terms of its ability to reduce the cytotoxicity of C. difficile supernatant. The composite part was effective in reducing toxin production by 85%.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None